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Marc Johnson

Marc Johnson

Professor, Molecular Microbiology and Immunology

Phone: 573-882-1519
marcjohnson@missouri.edu
471c Bond Life Sciences Center

Marc Johnson is an associate professor of molecular microbiology and immunology (MMI). The Johnson lab studies the interactions that occur between viruses (particularly HIV) and their hosts. Viruses are obligate intracellular parasites, meaning that they are completely unable replicate without the help of their host organism. They accomplish this by tricking cellular machinery into producing and assembling all of the pieces needed to make more virus. How this coercion is accomplished is often poorly understood. Often the normal role of host genes is only understood after we uncover how the virus subvert them. The Johnson lab in particular studies how viruses use host machinery to transport all of the viral pieces to correct location in the cell for assembly. A clearer understanding of these interactions will provide new avenues for the development of antiviral drugs.

News about Marc Johnson

Research Topics

2021

Structure of the mature Rous sarcoma virus lattice reveals a role for IP6 in the formation of the capsid hexamer. Obr M, Ricana CL, Nikulin N, Feathers J-PR, Klanschnig M, Thader A, Johnson MC, Vogt VM, Schur FKM, Dick RA. Nature Communications; 12(1):3226.

Date published: December 1, 2021
Featured in this article: Marc Johnson.

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Monitoring sars-cov-2 populations in wastewater by amplicon sequencing and using the novel program sam refiner. Gregory DA, Wieberg CG, Wenzel J, Lin C-H, Johnson MC. Viruses; 13(8):1647.

Date published: August 1, 2021
Featured in this article: Marc Johnson.

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SARS-CoV-2 show no infectivity at later stages in a prolonged COVID-19 patient despite positivity in RNA testing. Wan X-F, Tang CY, Ritter D, Wang Y, Li T, Segovia K, Kosikova M, Johnson M, Kwon HJ, Xie H, Hammer RD, McElroy JA. Journal of Medical Virology; 93(7):4570-4575.

Date published: July 1, 2021
Featured in this article: Henry Wan, Cynthia Tang, Yang Wang, Marc Johnson.

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2020

Optimized Pseudotyping Conditions for the SARS-COV-2 Spike Glycoprotein. Johnson MC, Lyddon TD, Suarez R, Salcedo B, LePique M, Graham M, Ricana C, Robinson C, Ritter DG. Journal of Virology; 94(21):e01062-20.

Date published: October 14, 2020
Featured in this article: Marc Johnson, Terri Lyddon.

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Primate lentiviruses require Inositol hexakisphosphate (IP6) or inositol pentakisphosphate (IP5) for the production of viral particles. Ricana CL, Johnson MC, Lyddon TD, Dick RA. PLoS Pathogens; 16(8):e1008646.

Date published: August 1, 2020
Featured in this article: Marc Johnson, Terri Lyddon.

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Structures of immature EIAV Gag lattices reveal a conserved role for IP6 in lentivirus assembly. Dick RA, Xu C, Morado DR, Kravchuk V, Ricana CL, Lyddon TD, Broad AM, Feathers JR, Johnson MC, Vogt VM, Perilla JR, Briggs JAG, Schur FKM. PLoS Pathogens; 16(1):e1008277.

Date published: January 27, 2020
Featured in this article: Terri Lyddon, Marc Johnson.

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2019

Diphtheria Toxin A-Resistant Cell Lines Enable Robust Production and Evaluation of DTA-Encoding Lentiviruses. Lange MJ, Lyddon TD, Johnson MC. Scientific Reports; 9(1):8985.

Date published: December 1, 2019
Featured in this article: Margaret Lange, Terri Lyddon, Marc Johnson.

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Sequence determinants in gammaretroviral Env cytoplasmic tails dictate virus-specific pseudotyping compatibility. Song YE, Olinger GY, Janaka SK, Johnson MC. Journal of Virology; 93(11):e0217218.

Date published: May 15, 2019
Featured in this article: Marc Johnson.

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Author Correction: A lipid-based partitioning mechanism for selective incorporation of proteins into membranes of HIV particles (Nature Cell Biology, (2019), 21, 4, (452-461), 10.1038/s41556-019-0300-Y). Sengupta P, Seo AY, Pasolli HA, Song YE, Johnson MC, Lippincott-Schwartz J. Nature Cell Biology; 21(5):662.

Date published: May 1, 2019
Featured in this article: Marc Johnson.

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A lipid-based partitioning mechanism for selective incorporation of proteins into membranes of HIV particles. Sengupta P, Seo AY, Pasolli HA, Song YE, Johnson MC, Lippincott-Schwartz J. Nature Cell Biology; 21(4):452-461.

Date published: April 1, 2019
Featured in this article: Marc Johnson.

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2018

Erratum to: Inositol phosphates are assembly co-factors for HIV-1 (Nature, (2018), 560, 7719, (509-512), 10.1038/s41586-018-0396-4). Dick RA, Zadrozny KK, Xu C, Schur FKM, Lyddon TD, Ricana CL, Wagner JM, Perilla JR, Ganser-Pornillos BK, Johnson MC, Pornillos O, Vogt VM. Nature; 563(7731):E22.

Date published: November 15, 2018
Featured in this article: Terri Lyddon, Marc Johnson.

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βTrCP is Required for HIV-1 Vpu Modulation of CD4, GaLV Env, and BST-2/Tetherin. Song YE, Cyburt D, Lucas TM, Gregory DA, Lyddon TD, Johnson MC. Viruses; 10(10):573.

Date published: October 19, 2018
Featured in this article: Terri Lyddon, Marc Johnson.

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Inositol phosphates are assembly co-factors for HIV-1. Dick RA, Zadrozny KK, Xu C, Schur FKM, Lyddon TD, Ricana CL, Wagner JM, Perilla JR, Ganser-Pornillos BK, Johnson MC, Pornillos O, Vogt VM. Nature; 560(7719):509-512.

Date published: August 23, 2018
Featured in this article: Terri Lyddon, Marc Johnson.

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2017

Sphingosine 1-phosphate lyase enhances the activation of IKK to promote type i IFN-mediated innate immune responses to influenza a virus infection. Vijayan M, Xia C, Song YE, Ngo H, Studstill CJ, Drews K, Fox TE, Johnson MC, Hiscott J, Kester M, Alexander S, Hahm B. Journal of Immunology; 199(2):677-687.

Date published: July 15, 2017
Featured in this article: Marc Johnson.

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RNA-protein interactions govern antiviral specificity and encapsidation of broad spectrum anti-HIV reverse transcriptase aptamers. Lange MJ, Nguyen PDM, Callaway MK, Johnson MC, Burke DH. Nucleic Acids Research; 45(10):6087-6097.

Date published: June 2, 2017
Featured in this article: Margaret Lange, Marc Johnson, Donald Burke-Agüero.

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2016

DHX9/RHA Binding to the PBS-Segment of the Genomic RNA during HIV-1 Assembly Bolsters Virion Infectivity. Boeras I, Song Z, Moran A, Franklin J, Brown WC, Johnson M, Boris-Lawrie K, Heng X. Journal of Molecular Biology; 428(11):2418-2429.

Date published: June 5, 2016
Featured in this article: Marc Johnson.

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In vivo analysis of infectivity, fusogenicity, and incorporation of a mutagenic viral glycoprotein library reveals determinants for virus incorporation. Salamango DJ, Alam KK, Burke DH, Johnson MC. Journal of Virology; 90(14):6502-6514.

Date published: May 4, 2016
Featured in this article: Donald Burke-Agüero, Marc Johnson.

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2015

Characterizing the murine leukemia virus envelope glycoprotein membrane-spanning domain for its roles in interface alignment and fusogenicity. Salamango DJ, Johnson MC. Journal of Virology; 89(24):12492-12500.

Date published: November 17, 2015
Featured in this article: Marc Johnson.

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2014

TIM-family proteins inhibit HIV-1 release. Li M, Ablan SD, Miao C, Zheng Y-M, Fuller MS, Rennert PD, Maury W, Johnson MC, Freed EO, Liu S-L. Proceedings of the National Academy of Sciences of the United States of America; 111(35):E3699-E3707.

Date published: September 2, 2014
Featured in this article: Marc Johnson.

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Diverse viral glycoproteins as well as CD4 co-package into the same human immunodeficiency virus (HIV-1) particles. Gregory DA, Olinger GY, Lucas TM, Johnson MC. Retrovirology; 11(1):28.

Date published: March 3, 2014
Featured in this article: Marc Johnson.

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2013

Recombination can lead to spurious results in retroviral transduction with dually fluorescent reporter genes. Salamango DJ, Evans DA, Baluyot MF, Furlong JN, Johnson MC. Journal of Virology; 87(24):13900-13903.

Date published: December 1, 2013
Featured in this article: Marc Johnson.

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Retrovirus glycoprotein functionality requires proper alignment of the ectodomain and the membrane-proximal cytoplasmic tail. Janaka SK, Gregory DA, Johnson MC. Journal of Virology; 87(23):12805-12813.

Date published: December 1, 2013
Featured in this article: Marc Johnson.

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Functional Complementation of a Model Target to Study Vpu Sensitivity. Janaka SK, Faurot J, Johnson MC. PLoS ONE; 8(6):e68507.

Date published: June 28, 2013
Featured in this article: Marc Johnson.

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Sequences in gibbon ape leukemia virus envelope that confer sensitivity to HIV-1 accessory protein Vpu (Erratum). Janaka SK, Lucas TM, Johnson MC. Journal of Virology; 87(12):7198.

Date published: June 1, 2013
Featured in this article: Marc Johnson.

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Multiple gag domains contribute to selective recruitment of murine leukemia virus (MLV) Env to MLV virions. Gregory DA, Lyddon TD, Johnson MC. Journal of Virology; 87(3):1518-1527.

Date published: February 1, 2013
Featured in this article: Terri Lyddon, Marc Johnson.

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2012

Vpu Downmodulates Two Distinct Targets, Tetherin and Gibbon Ape Leukemia Virus Envelope, through Shared Features in the Vpu Cytoplasmic Tail. Lucas TM, Janaka SK, Stephens EB, Johnson MC. PLoS ONE; 7(12):e51741.

Date published: December 19, 2012
Featured in this article: Marc Johnson.

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Robust suppression of HIV replication by intracellularly expressed reverse transcriptase aptamers is independent of ribozyme processing. Lange MJ, Sharma TK, Whatley AS, Landon LA, Tempesta MA, Johnson MC, Burke DH. Molecular Therapy; 20(12):2304-2314.

Date published: December 1, 2012
Featured in this article: Margaret Lange, Marc Johnson, Donald Burke-Agüero.

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CRM1-dependent trafficking of retroviral Gag proteins Revisited. Baluyot MF, Grosse SA, Lyddon TD, Janaka SK, Johnson MC. Journal of Virology; 86(8):4696-4700.

Date published: April 1, 2012
Featured in this article: Terri Lyddon, Marc Johnson.

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Biochemical, inhibition and inhibitor resistance studies of xenotropic murine leukemia virus-related virus reverse transcriptase. Ndongwe TP, Adedeji AO, Michailidis E, Ong YT, Hachiya A, Marchand B, Ryan EM, Rai DK, Kirby KA, Whatley AS, Burke DH, Johnson M, Ding S, Zheng Y-M, Liu S-L, Kodama E-I, Delviks-Frankenberry KA, Pathak VK, Mitsuya H, Parniak MA, Singh K, Sarafianos SG. Nucleic Acids Research; 40(1):345-359.

Date published: January 1, 2012
Featured in this article: Donald Burke-Agüero, Marc Johnson, Kamlendra Singh.

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2011

Sequences in gibbon ape leukemia virus envelope that confer sensitivity to HIV-1 accessory protein Vpu. Janaka SK, Lucas TM, Johnson MC. Journal of Virology; 85(22):11945-11954.

Date published: November 1, 2011
Featured in this article: Marc Johnson.

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Clathrin facilitates the morphogenesis of retrovirus particles. Zhang F, Zang T, Wilson SJ, Johnson MC, Bieniasz PD. PLoS Pathogens; 7(6):e1002119.

Date published: June 1, 2011
Featured in this article: Marc Johnson.

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Mechanisms for env glycoprotein acquisition by retroviruses. Johnson MC. AIDS Research and Human Retroviruses; 27(3):239-247.

Date published: March 1, 2011
Featured in this article: Marc Johnson.

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2010

Two distinct mechanisms regulate recruitment of murine leukemia virus envelope protein to retroviral assembly sites. Lucas TM, Lyddon TD, Grosse SA, Johnson MC. Virology; 405(2):548-555.

Date published: September 30, 2010
Featured in this article: Terri Lyddon, Marc Johnson.

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An LYPSL late domain in the Gag protein contributes to the efficient release and replication of Rous sarcoma virus. Dilley KA, Gregory D, Johnson MC, Vogt VM. Journal of Virology; 84(13):6276-6287.

Date published: July 1, 2010
Featured in this article: Marc Johnson.

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Pseudotyping incompatibility between HIV-1 and gibbon ape leukemia virus Env is modulated by Vpu. Lucas TM, Lyddon TD, Cannon PM, Johnson MC. Journal of Virology; 84(6):2666-2674.

Date published: March 1, 2010
Featured in this article: Terri Lyddon, Marc Johnson.

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2009

Tetherin Inhibits HIV-1 Release by Directly Tethering Virions to Cells. Perez-Caballero D, Zang T, Ebrahimi A, McNatt MW, Gregory DA, Johnson MC, Bieniasz PD. Cell; 139(3):499-511.

Date published: October 30, 2009
Featured in this article: Marc Johnson.

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Nef Proteins from Simian Immunodeficiency Viruses Are Tetherin Antagonists. Zhang F, Wilson SJ, Landford WC, Virgen B, Gregory D, Johnson MC, Munch J, Kirchhoff F, Bieniasz PD, Hatziioannou T. Cell Host and Microbe; 6(1):54-67.

Date published: July 23, 2009
Featured in this article: Marc Johnson.

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Foreign glycoproteins can be actively recruited to virus assembly sites during pseudotyping. Jorgenson RL, Vogt VM, Johnson MC. Journal of Virology; 83(9):4060-4067.

Date published: May 1, 2009
Featured in this article: Marc Johnson.

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2008

Three-Dimensional Analysis of Budding Sites and Released Virus Suggests a Revised Model for HIV-1 Morphogenesis. Carlson L-A, Briggs JAG, Glass B, Riches JD, Simon MN, Johnson MC, Müller B, Grünewald K, Kräusslich H-G. Cell Host and Microbe; 4(6):592-599.

Date published: December 11, 2008
Featured in this article: Marc Johnson.

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Mutations in the spacer peptide and adjoining sequences in rous sarcoma virus gag lead to tubular budding. Keller PW, Johnson MC, Vogt VM. Journal of Virology; 82(14):6788-6797.

Date published: July 1, 2008
Featured in this article: Marc Johnson.

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The Interferon-Induced Protein BST-2 Restricts HIV-1 Release and Is Downregulated from the Cell Surface by the Viral Vpu Protein. Van Damme N, Goff D, Katsura C, Jorgenson RL, Mitchell R, Johnson MC, Stephens EB, Guatelli J. Cell Host and Microbe; 3(4):245-252.

Date published: April 17, 2008
Featured in this article: Marc Johnson.

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Neural cell adhesion molecule (NCAM) marks adult myogenic cells committed to differentiation. Capkovic KL, Stevenson S, Johnson MC, Thelen JJ, Cornelison DDW. Experimental Cell Research; 314(7):1553-1564.

Date published: April 15, 2008
Featured in this article: Marc Johnson, Jay Thelen, D Cornelison.

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2007

Ubiquitin-dependent virus particle budding without viral protein ubiquitination. Zhadina M, McClure MO, Johnson MC, Bieniasz PD. Proceedings of the National Academy of Sciences of the United States of America; 104(50):20031-20036.

Date published: December 11, 2007
Featured in this article: Marc Johnson.

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Probing protein adsorption onto mercaptoundecanoic acid stabilized gold nanoparticles and surfaces by quartz crystal microbalance and ζ-potential measurements. Kaufman ED, Belyea J, Johnson MC, Nicholson ZM, Ricks JL, Shah PK, Bayless M, Pettersson T, Feldotö Z, Blomberg E, Claesson P, Franzen S. Langmuir; 23(11):6053-6062.

Date published: May 22, 2007
Featured in this article: Marc Johnson.

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The C-terminal portion of the Hrs protein interacts with Tsg101 and interferes with human immunodeficiency virus type 1 Gag particle production. Bouamr F, Houck-Loomis BR, De Los Santos M, Casaday RJ, Johnson MC, Goff SP. Journal of Virology; 81(6):2909-2922.

Date published: March 1, 2007
Featured in this article: Marc Johnson.

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2006

Plasma membrane is the site of productive HIV-1 particle assembly. Jouvenet N, Neil SJD, Bess C, Johnson MC, Virgen CA, Simon SM, Bieniasz PD. PLoS Biology; 4(12):2296-2310.

Date published: December 1, 2006
Featured in this article: Marc Johnson.

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Cryo-electron Microscopy Reveals Conserved and Divergent Features of Gag Packing in Immature Particles of Rous Sarcoma Virus and Human Immunodeficiency Virus. Briggs JAG, Johnson MC, Simon MN, Fuller SD, Vogt VM. Journal of Molecular Biology; 355(1):157-168.

Date published: January 6, 2006
Featured in this article: Marc Johnson.

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2005

The retrovirai capsid domain dictates virion size, morphology, and coassembly of Gag into virus-like particles. Ako-Adjei D, Johnson MC, Vogt VM. Journal of Virology; 79(21):13463-13472.

Date published: November 1, 2005
Featured in this article: Marc Johnson.

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Visualization of retrovirus budding with correlated light and electron microscopy. Larson DR, Johnson MC, Webb WW, Vogt VM. Proceedings of the National Academy of Sciences of the United States of America; 102(43):15453-15458.

Date published: October 25, 2005
Featured in this article: Marc Johnson.

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The C-terminal half of TSG101 blocks rous sarcoma virus budding and sequesters gag into unique nonendosomal structures. Johnson MC, Spidel JL, Ako-Adjei D, Wills JW, Vogt VM. Journal of Virology; 79(6):3775-3786.

Date published: March 1, 2005
Featured in this article: Marc Johnson.

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2004

The stoichiometry of Gag protein in HIV-1. Briggs JAG, Simon MN, Gross I, Kräusslich H-G, Fuller SD, Vogt VM, Johnson MC. Nature Structural and Molecular Biology; 11(7):672-675.

Date published: July 1, 2004
Featured in this article: Marc Johnson.

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The NV gene of snakehead rhabdovirus (SHRV) is not required for pathogenesis, and a heterologous glycoprotein can be incorporated into the SHRV envelope. Alonso M, Kim CH, Johnson MC, Pressley M, Leong J-A. Journal of Virology; 78(11):5875-5882.

Date published: June 1, 2004
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Cloning and characterization of an Mx gene and its corresponding promoter from the zebrafish, Danio rerio. Altmann SM, Mellon MT, Johnson MC, Paw BH, Trede NS, Zon LI, Kim CH. Developmental and Comparative Immunology; 28(4):295-306.

Date published: April 1, 2004
Featured in this article: Marc Johnson.

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Dimeric Rous sarcoma virus capsid protein structure relevant to immature gag assembly. Nandhagopal N, Simpson AA, Johnson MC, Francisco AB, Schatz GW, Rossmann MG, Vogt VM. Journal of Molecular Biology; 335(1):275-282.

Date published: January 2, 2004
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2003

A fish specific expression vector containing the interferon regulatory factor 1A (IRF1A) promoter for genetic immunization of fish. Alonso M, Johnson M, Simon B, Leong J-A. Vaccine; 21(15):1591-1600.

Date published: April 2, 2003
Featured in this article: Marc Johnson.

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2002

Nucleic acid-independent retrovirus assembly can be driven by dimerization. Johnson MC, Scobie HM, Ma YM, Vogt VM. Journal of Virology; 76(22):11177-11185.

Date published: November 15, 2002
Featured in this article: Marc Johnson.

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2001

PR domain of rous sarcoma virus gag causes an assembly/budding defect in insect cells. Johnson MC, Scobie HM, Vogt VM. Journal of Virology; 75(9):4407-4412.

Date published: May 1, 2001
Featured in this article: Marc Johnson.

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A GP64-null baculovirus pseudotyped with vesicular stomatitis virus G protein. Mangor JT, Monsma SA, Johnson MC, Blissard GW. Journal of Virology; 75(6):2544-2556.

Date published: March 15, 2001
Featured in this article: Marc Johnson.

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2000

DNA vaccines encoding viral glycoproteins induce nonspecific immunity and Mx protein synthesis in fish. Kim CH, Johnson MC, Drennan JD, Simon BE, Thomann E, Leong J-A. Journal of Virology; 74(15):7048-7054.

Date published: August 1, 2000
Featured in this article: Marc Johnson.

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Production of recombinant Snakehead rhabdovirus: The NV protein is not required for viral replication. Johnson MC, Simon BE, Kim CH, Leong J-AC. Journal of Virology; 74(5):2343-2350.

Date published: March 1, 2000
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1999

Molecular characterization of the glycoproteins from two warm water rhabdoviruses: Snakehead rhabdovirus (SHRV) and rhabdovirus of penaeid shrimp (RPS)/spring viremia of carp virus (SVCV). Johnson MC, Maxwell JM, Loh PC, Leong J-AC. Virus Research; 64(2):95-106.

Date published: November 1, 1999
Featured in this article: Marc Johnson.

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1998

Interferon-inducible Mx proteins in fish. Leong J-AC, Trobridge GD, Kim CHY, Johnson M, Simon B. Immunological Reviews; 166:349-363.

Date published: January 1, 1998
Featured in this article: Marc Johnson.

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1997

Fish vaccine antigens produced or delivered by recombinant DNA technologies. Leong JC, Anderson E, Bootland LM, Chiou PW, Johnson M, Kim C, Mourich D, Trobridge G. Developments in biological standardization; 90:267-277.

Date published: January 1, 1997
Featured in this article: Marc Johnson.